CD9 antibody (Extracellular)

Catalog No. ABIN7581848

The Rabbit Polyclonal anti-CD9 antibody has been validated for FACS, WB, IHC and LCI. It is suitable to detect CD9 in samples from Mouse.

Quick Overview for CD9 antibody (Extracellular) (ABIN7581848)

Target: CD9

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Product Details anti-CD9 Antibody

Binding Specificity: AA 114-129, Extracellular

Purpose: A Rabbit Polyclonal antibody to CD9 (extracellular)

Sequence: (C)EVIKELQEFY KDTYQK

Specificity: Extracellular, 2nd loop.

Predicted Reactivity: Rat - identical,Human - 14 out of 16 amino acid residues identical

Characteristics: Anti-CD9 (extracellular) Antibody (ABIN7581848) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CD9 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EVIKELQEFYKDTYQK, corresponding to amino acid residues 114 - 129 of mouse CD9 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CD9

Target: CD9

Alternative Name: CD9

Background: Tetrapanin-29, TSPAN29, Leukocyte Antigen MIC3, MIC3,Cluster of Differentiation 9 (CD9), also known as Tetrapanin-29, TSPAN29 and Leukocyte Antigen MIC3, is a member of the tetraspanin superfamily, a group of proteins characterized by four transmembrane domains, two extracellular loops, and N- and C- termini cytoplasmic tails.1The tetraspanins family members function as organizers of the cell surface by recruiting specific partner proteins into tetraspanin-enriched microdomains. Thus, depending on the recruited protein, they influence many cellular activities in different cells, including intracellular signaling, proliferation, activation, survival, migration, invasion, adhesion, and diapedesis.1CD9 was originally identified as a lymphohematopoietic marker, and is indeed expressed in all the major subsets of leukocytes, however, it is also widely expressed across different cell populations including endothelial cells, neurons, glia and oocytes.2,3CD9 plays various roles in the immune system, it has been implicated in T cell activation and participating in the formation of immunological synapses by interacting with the integrin LFA-1 (on the T-cell side) and their ligand, the integrin ICAM-1 on the antigen presenting cell (APC) surface.2,3 In B cells, CD9 plays a role in B cell activation and regulation of antibody production, and in mast cells is involved in the regulation of signalling pathways that influence the release of allergic mediators in response to allergens.2,3CD9 has complex roles in the central nervous system (CNS). It has been implicated in neurite outgrowth and cell migration in association with the neural adhesion molecule L1 (L1CAM). It is also involved in myelination and the maintaining of the myelin sheath, as well as the regulation of neural progenitor cells.4Finally, CD9 is often associated with exosomes, small extracellular vesicles released by cells that play a crucial role in intercellular communication by carrying proteins, lipids, and nucleic acids between cells. CD9 is considered a marker for exosomes and detection of CD9 on the surface of extracellular vesicles is often used as a method to isolate and identify exosomes from biological fluids or cell culture supernatants.5,6

Gene ID: 12527

UniProt: P40240

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization